Plasmid

Part:BBa_K2061000:Design

Designed by: avi matityahu   Group: iGEM16_Tel-Hai   (2016-08-23)


tGFP ORF under EF1a promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 541
    Illegal XhoI site found at 939
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
    Illegal AgeI site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1365


Design Notes

the part cloned between EcoRI and XbaI sites.


Source

synthetic production of the part

References